Principles of Fluorescence Spectroscopy

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About this book

Principles of Fluorescence Spectroscopy, 3 rd edition

Joseph R. Lakowicz

The third edition of the established classic text reference, Principles of Fluorescence Spectroscopy, will enhance upon the earlier editions' successes. Organized as a textbook for the learning student or the researcher needing to acquire the core competencies, Principles of Fluorescence Spectroscopy, 3e will maintain the emphasis on basics, while updating the examples to include recent results from the literature. The third edition also includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering.

This full-color textbook features the following:

Principles of Fluorescence Spectroscopy, 3 rd edition, is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

About the Author:

Dr. Joseph R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine, Baltimore, and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is theauthor of the widely used text, Principles of Fluorescence Spectroscopy now in its 3 rd edition.

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Table of contents (26 chapters)

Front Matter

Pages i-xxvi

Introduction to Fluorescence

Instrumentation for Fluorescence Spectroscopy

Pages 27-61

Fluorophores

Pages 63-95

Time-Domain Lifetime Measurements

Pages 97-155

Frequency-Domain Lifetime Measurements

Pages 157-204

Solvent and Environmental Effects

Pages 205-235

Dynamics of Solvent and Spectral Relaxation

Pages 237-276

Quenching of Fluorescence

Pages 277-330

Mechanisms and Dynamics of Fluorescence Quenching

Pages 331-351

Fluorescence Anisotropy

Pages 353-382

Time-Dependent Anisotropy Decays

Pages 383-412

Advanced Anisotropy Concepts

Pages 413-441

Energy Transfer

Pages 443-475

Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers

Pages 477-506

Energy Transfer to Multiple Acceptors in One,Two, or Three Dimensions

Pages 507-528

Protein Fluorescence

Pages 529-575

Time-Resolved Protein Fluorescence

Pages 577-606

Multiphoton Excitation and Microscopy

Pages 607-621

Fluorescence Sensing

Pages 623-673

Reviews

Praise for Earlier Editions:

"Lakowicz’s Principles of Fluorescence Spectroscopy has been the best one-volume introduction to the biophysical principles of fluorescence methods.

- Roger Y. Tsien, Ph.D., Department of Pharmacology and Department of Chemistry and Biochemistry, University of California, San Diego, California

" Principles of Fluorescence Spectroscopy is encyclopedic and comprehensive."

- Britton Chance, Professor Emeritus in Biochemistry and Biophysics,University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania

"Recommended without reservation both to the novice and to the expert in fluorescence."

"In addition to its use as a student text, it should be a particularly valuable reference for those involved in biochemical research."

- Chemistry in Britain

Advance Praise for Third Edition:

"This third edition has significantly expanded the topics, and will remain as a leading reference, as well as a text…the information in the book is valuable for a wide range of disciplines."

- Robert M. Clegg, Ph.D., Department of Physics, University of Illinois, Champaign-Urbana, Illinois

"Overall this is a most welcome, and timely transformation of the classic, and most comprehensive textbook on fluorescence spectroscopy. It should be the number one item on the shopping list for any student or researcher involved in any aspect of fluorescence, be it as a biologist who does some microscopy, or a chemist synthesizing novel fluorophores."

- Alan Ryder, Ph.D., National Centre for Biomedical Engineering Science, National University of Ireland-Galway, Galway, Ireland

From the reviews of the third edition:

"This book gives an overview of the principles and applications of fluorescence. It is wellstructured, starting with basic knowledge about the phenomena of fluorescence and ending with the latest applications. … highly readable and informative both by novices and by experienced people. … a helpful work of reference and a wonderful creation for learning and teaching. The updated 3rd edition with its appealing design and its absolutely up-to-date and, nevertheless, complete treatment of fluorescence spectroscopy makes it essential for everyone working in this field." (Christiane Albrecht, Analytical and Bioanalytical Chemistry, Vol. 390, 2008)

Editors and Affiliations

Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, USA

About the editor

Dr. Joseph R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine, Baltimore, and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is the author of the widely used text, Principles of Fluorescence Spectroscopy now in its 3 rd edition.

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